Effects of Reactive Oxygen Species in p16 Expression and its Modulation by Curcumin in MCF-7 Breast Cancer Cell Line

Abstract

Cancer is a group disease involving abnormal cell growth with the potential spread to other parts of the body.
Cancer is also known as a malignant tumor (National cancer institute 2014). A neoplasm or tumor is a group
of cells that have undergone unregulated growth, and will often form a mass or lump, but may be distributed
diffusely. The genetic changes that contribute to cancer tend to affect three main types of genes- proto-oncogenes,
tumor suppressor genes and DNA repair genes
Objectives: To investigate the role of p16 protein expression and to see the effect of curcumin in ROS affected
MCF-7 cells by western blotting.
Keeping the background in mind, the present study were designed to analysis the role of p16 protein expression
and to see the effect of curcumin in ROS affected MCF-7 cells by western blotting. Also see the possible cellular
mechanisms by evaluating cell cycle distribution and intracellular ROS generation.